FastQCFastQC Report
Thu 28 Apr 2016
ERR1041554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066273
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238170.4701088946450379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200510.3957741716642589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT171810.339125033333182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83630.16507203618912758No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT57360.11321932315925337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG13200.018.2196965
GACGGAC12600.017.9126997
ACGGACC13600.016.8676478
CTAGCGG7850.016.73248529
CAAGACG15450.016.6440124
TCTAGCG7950.016.52201528
CGGACCA14450.016.003469
CGCAAGA14400.015.9305552
AGACGGA14450.015.7474046
TACCGTC7700.015.6168827
GGTATCA100450.015.433551
CGAGCCG15600.015.29807815
TCGTTTA7200.015.15972230
TACGACG8450.015.1065085
CGACGGT8300.014.9337357
ACGACGG8450.014.8875736
ACCGTCG7600.014.8486848
GCGCAAG15950.014.84639551
CGTCGTA7450.014.40268410
CGCTATA908.28088E-414.3888882
Produced by FastQC (version 0.11.5)