FastQCFastQC Report
Thu 28 Apr 2016
ERR1041553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9242524
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT364040.3938750929940783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT290220.31400513539375174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266500.2883411501014225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128210.13871751915385885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG11150.017.75336529
GACGGAC15900.017.452837
AAGACGG16900.017.4053275
TCTAGCG11500.016.89130428
ACGGACC16600.016.8283148
CGCAAGA16850.016.4688432
AGACGGA18650.015.5737286
CTAACGC1557.228664E-915.51612953
CGGACCA18550.015.159039
TATACTG17650.014.9886685
GCGCAAG21000.014.44761851
TAACGGC6550.014.40457936
GTATTAG33650.014.3491841
CGCAATA13800.014.34420236
TTAACGG6500.014.23076935
CGTTCGG3600.013.87500124
CGTAGCG3800.013.63157915
TAGGACG8700.013.6091964
ATCGATA6100.013.34426123
GTATAGG20450.013.2078241
Produced by FastQC (version 0.11.5)