FastQCFastQC Report
Thu 28 Apr 2016
ERR1041552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8420315
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT311550.36999803451533586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT247000.2933381945924826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218360.2593252152680749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106660.12666984548677812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG10700.016.25233729
CGCAATA11900.015.85714336
TCTAGCG11350.015.32158628
GGTATCA145950.015.1980131
ACGGACC16100.015.0527958
GACGGAC16350.015.0489297
AAGACGG18750.014.8986665
TTAACGG3800.014.11842235
GTAAACG9800.013.78061227
TAACGCC11300.013.5884954
CGCAAGA18700.013.5534762
CGAACGA7550.013.47682216
AGACGGA19450.013.4113116
GCGCAAG20600.013.3810681
ACGAACG7650.013.30065315
CGGACCA18900.013.1164039
ATAATAC16250.013.0923073
TATACTG18100.012.9806625
TAAGTCG2001.4666512E-812.94999913
TAGCGGC14250.012.85263230
Produced by FastQC (version 0.11.5)