FastQCFastQC Report
Thu 28 Apr 2016
ERR1041551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15534052
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT542520.34924564434315014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT410230.26408434837220834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT388080.24982535142794682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185910.11967901227574107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198300.019.6381761
AAGACGG31050.017.4573275
ACGGACC29300.017.0477838
GACGGAC30100.016.5946857
ACCGTCG19850.016.2166258
TACCGTC21650.015.6374147
TCTAGCG19550.015.14066528
CTAGCGG19350.015.10594429
CGCAAGA35500.015.00845052
ATACCGT25650.015.001956
TAACGCC21050.014.8527334
CGTCGTA20750.014.71084310
CCGTCGT21200.014.66037759
CGGACCA35650.014.2706869
AGACGGA36000.014.2347226
TATACTG33200.014.1536155
ATAACGC24300.014.0843633
GCGCAAG39000.013.993591
CAAGACG40250.013.9726714
GTATCAA284500.013.6295252
Produced by FastQC (version 0.11.5)