FastQCFastQC Report
Thu 28 Apr 2016
ERR1041550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691346
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196880.255976002119785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT150340.19546643721398047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT146210.19009676589767252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.022.1313761
GACGGAC11300.015.5530977
ACGGACC12100.014.8305788
ACGAACG6750.014.52592615
AAGACGG14200.014.4612675
CGAACGA6550.014.4045816
CGACGGT7600.014.3618427
GTATCAA101100.014.1266082
ACGACGG8100.013.7037046
TACGACG8450.013.5739645
TAACGCC9600.013.2968764
GTAATAC9400.013.1861713
ATAACGC11600.013.0775863
CGCAAGA14700.012.8367352
AGACGGA14650.012.6279866
CGGACCA14200.012.5070429
GTATAGG10800.012.5046291
ATACCGC15400.012.49350627
TCGTTTA9800.012.45918430
CCTATAC9400.012.2021283
Produced by FastQC (version 0.11.5)