FastQCFastQC Report
Thu 28 Apr 2016
ERR1041549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752601
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT398370.5138533506367734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT296730.3827489638638697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT282250.3640713613405359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123130.15882411593218845No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87860.11332970702348799No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT81230.10477773846480684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC21000.018.3238097
AAGACGG22100.017.9977385
ACGGACC21200.017.889158
CAAGACG23300.017.4678124
CGCAAGA24650.016.2860032
TCTAGCG14350.016.24390428
CGGACCA24000.015.956259
AGACGGA24350.015.9548246
CTAGCGG15500.015.2774229
CGCAATA15550.014.99035536
GCGCAAG26750.014.7308421
GGTATCA198000.014.6411611
CGAACGA8500.014.36470616
GCGAAAG26850.014.19366818
GAGCGAA27300.014.09523816
ACGAACG8450.014.01183515
CGAGCCG24550.013.86558115
GCAAGAC36150.013.71507553
CGAAAGC27600.013.67391219
TCTATAC11550.013.6147183
Produced by FastQC (version 0.11.5)