FastQCFastQC Report
Thu 28 Apr 2016
ERR1041548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8616545
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT394270.4575731920392686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT282920.32834506173878275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT278730.3234823238316518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111680.12961111443159642No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT97170.11277141824246262No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT93680.10872107091647522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC22800.019.5548258
GACGGAC23600.019.0487297
AAGACGG25250.018.8297045
CAAGACG27600.017.3605084
CGCAAGA26050.017.1861822
CGGACCA26300.016.952479
AGACGGA27000.016.7870376
GCGCAAG28650.015.9493891
GCGAAAG28750.015.3791318
CTAGCGG16100.015.28260929
CGAGCCG25900.015.21428715
TCGTTTA18200.015.04395630
GAGCGAA30100.014.68936916
GCAAGAC37750.014.6529813
TCTAGCG16700.014.62275428
CGAAAGC30050.014.59068319
CGCAATA17200.014.52034936
TACCGTC15950.014.3824447
AGCGAAA30550.014.35188217
ACCGTCG15200.014.2401318
Produced by FastQC (version 0.11.5)