FastQCFastQC Report
Thu 28 Apr 2016
ERR1041547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704886
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99700.5847898334551401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72680.42630416344553246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69080.4051883820971021No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.1699233849066741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAG250.005497080329.59999828
TCTAGCG3050.018.19672228
CGCAATA3050.018.19672236
ATTAGAC1502.5102054E-1017.2666663
TAGCGGC3350.017.11940430
CTAGCGG3250.017.07692329
TTAGACT2000.016.654
CGTCCCG904.4479842E-516.44444510
GCGCAAT3450.016.08695635
TCTAGAC2200.015.9772733
TCGTTTA2550.015.96078430
GGGTTAG1659.749783E-1015.6969717
GGGGTTA1802.0190782E-1015.4166676
TAACGCC2800.015.1964284
CGACGGT2550.014.5098047
GGGTATA908.276905E-414.3888891
TAGCCCG908.276905E-414.3888895
TTATACT3650.014.1917814
GACGGAC4050.014.1604947
GTAATAC1702.4385372E-814.1470593
Produced by FastQC (version 0.11.5)