FastQCFastQC Report
Thu 28 Apr 2016
ERR1041546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140398
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35260.30919030022851673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.29936916760639704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23860.20922520032479888No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12170.10671712858142507No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG11970.10496335489890371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG705.101865E-621.14285735
GTATACG450.003825789820.5555551
TCTAGCG1355.638867E-1119.18518428
TCGTTTA2100.018.530
GCGTGCG902.1521864E-618.531
TAACGCC2300.018.4999984
CTAGTAC500.007034850318.4999983
ATAACGC2550.018.1372553
AACGCCG2150.018.0697675
CGCATCG2050.018.0487813
CGAGCCG2900.017.86206815
TGGTCGG2400.017.72916637
CGTGCGA852.7230855E-517.41176432
TGTACCG1502.5102054E-1017.2666665
CTAGCGG1502.5102054E-1017.26666629
CGAACGT650.00157992417.0769234
GGACCGT650.00157992417.0769236
CAAGACG3150.017.0317464
GTAAACG1757.2759576E-1216.91428627
TCGCCGG2300.016.89130417
Produced by FastQC (version 0.11.5)