FastQCFastQC Report
Thu 28 Apr 2016
ERR1041545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371150
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77630.5661670860226816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64870.4731065164278161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54730.3991539948218648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.216825292637567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1750.022.19999910
TACCGTC1700.021.7647067
TACACCG951.676708E-719.4736845
CCGTCGT2000.019.4259
ATACCGT2000.019.4256
ACCGTCG1950.018.974368
GGACCGT609.238053E-418.56
GGTCTAG801.6170408E-518.51
TAATACG500.00703555818.4999984
AAGACGG2750.018.1636375
TATACTG2250.018.0888885
TAGGACG650.001580144417.0769234
ACGGACC2800.016.5178578
GACGGAC2850.016.228077
AGACGGA3150.015.8571426
GTATAGG2100.015.8571421
ACGAACG1302.5898225E-715.65384615
CCTACAC2055.456968E-1215.3414633
TCTAGAT2201.8189894E-1215.1363642
TCTAGAC1101.4521953E-515.1363643
Produced by FastQC (version 0.11.5)