FastQCFastQC Report
Thu 28 Apr 2016
ERR1041544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665172
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148860.5585380605829567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129980.48769835492793706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102510.38462808404110505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57250.21480789982785353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC4200.019.380957
ACGGACC4400.018.9204548
TACGACT852.7252974E-517.4117664
AAGACGG5000.017.025
CAAGACG5800.016.267244
TCGTTTA3900.015.65384530
CGCAAGA5700.015.5789472
CTAATCG1455.3538315E-815.31034526
GTCTTAC2300.015.2826071
CGTTATT3400.015.2352952
TAAACGC2450.015.1020428
TATACTG6200.014.9193555
CGAACGA1904.5656634E-1014.60526416
GTACGAC908.279061E-414.3888893
CGGACCA6050.014.37190159
ATGGTCG4000.014.33749936
TAATACT6200.014.3225814
TCTATAC4150.014.265063
TCTAGCG3400.014.14705928
ATCGCCG4450.014.13483216
Produced by FastQC (version 0.11.5)