FastQCFastQC Report
Thu 28 Apr 2016
ERR1041543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807749
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94000.5199836924263268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88950.4920483983119338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66030.3652608852224507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31380.17358604540785252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT451.3236221E-424.66666611
GCGTTAA400.001931612523.1250021
TCGTTTA2400.020.812530
ATCGATA450.003826643820.55555523
CGCATCG2750.018.16363713
CCTATAC2400.017.7291683
TAACGGC650.001580407117.07692336
TTAACGG650.001580407117.07692335
CGTTTAT3050.016.98360631
GGCGTTA1201.0421536E-716.95833431
ATCGTTT2850.016.87719329
TAACGCC3100.016.7096794
GACGGAC4650.016.7096797
GTATAGA3100.016.7096791
ATCGCCG3100.016.70967916
TCGCCGG3000.016.6517
CGCAAGA5150.016.5242732
AAGACGG5200.016.3653855
ACGGACC4650.016.3118298
CCGGTCG2750.016.14545420
Produced by FastQC (version 0.11.5)