FastQCFastQC Report
Thu 28 Apr 2016
ERR1041542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036373
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176040.5797706671742899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153020.5039565297148935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126210.41566039481974054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65510.21575083166659698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT3950.017.7974681
GACGGAC6150.017.7479677
CGTTATT3450.017.159422
ACGGACC6800.016.0514728
AGACGGA7150.016.041966
AAGACGG7700.015.8571425
ACGAACG2250.015.62222215
CTAGTAC2055.456968E-1215.3414643
TCGTTTA4600.015.28260930
CGCAAGA7450.015.1476512
TACGTAG1101.45335125E-515.13636414
TAACGCC4800.015.0312514
CGATGCG4350.014.88505833
CGAACGA2251.8189894E-1214.816
CGCATCG4500.014.813
TCTAGCG4250.014.828
CTAGCGG4200.014.53571429
GCGCAAG7900.014.5189871
GGTATCA78950.014.504751
GTATTAG8350.014.4011971
Produced by FastQC (version 0.11.5)