FastQCFastQC Report
Thu 28 Apr 2016
ERR1041541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649364
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112780.42568707055731114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99320.3748824246120956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82650.31196166325201063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36700.13852381175255646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG806.967184E-720.812535
AAGACGG7100.017.4577475
ACGGACC6700.017.3955238
GGTATCA44350.017.3528751
GACGGAC6750.016.9925927
CGCAAGA7350.016.8639452
CGCTATA1005.883616E-616.6500022
ACGCTTC3700.016.531
GTAAACG3500.016.38571527
GTTAGAC1151.2436158E-616.0869563
AGACGGA7750.015.9935476
TAAACGC3750.015.78666628
TATACTG5650.015.7168135
TCGTTTA4250.015.670588530
CGGACCA7550.015.4370879
TCTAGCG3150.015.26984228
CAAGACG8300.014.7108434
ATTGACG6300.014.6825432
TCTATAC3800.014.6052643
GCGCAAG8150.014.5276091
Produced by FastQC (version 0.11.5)