FastQCFastQC Report
Thu 28 Apr 2016
ERR1041540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739600
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108830.6256035870315014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT107070.6154863186939526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61160.35157507472982297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.20970338008737638No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18170.10444929868935389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG4650.020.6881715
CGCATCG3300.019.62121213
ACGGACC4450.019.5393268
TCGTTTA3400.018.530
GACGGAC4700.018.4999987
CAAGACG5450.018.3302754
TAACGCC3750.017.7600024
CCGGTCG3650.017.73972720
AGACGGA5050.017.584166
TTAACGG953.608542E-617.52631635
CGGACCA4750.017.5263169
CGTTTAT3500.017.44285631
CGCAAGA5150.017.242722
GCGCAAG5600.017.1785721
AATAACG3800.017.0394742
ATCGCCG4000.016.65000216
GCATCGC3900.016.60256414
GCGAAAG5250.016.20952418
CATCGTT3800.016.0657928
ATAACGC4150.016.0481933
Produced by FastQC (version 0.11.5)