FastQCFastQC Report
Thu 28 Apr 2016
ERR1041539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5505791
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT223040.4051007384769963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188250.34191272425705954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT154170.28001426134773366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77950.14157820374947033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79750.018.9291531
ACGGACC9900.017.005058
CTAGCGG7750.016.23225829
AAGACGG11400.016.228075
GACGGAC10450.015.9330167
ACGAACG5600.015.85714215
TATATCG3050.015.7704915
TCTAGCG8250.015.6969728
CGACGGT8800.015.5568187
CGAACGA5750.015.44347916
CGCAATA8550.015.14619936
ACGACGG9100.015.0439576
TACGACG9000.015.0055565
ATAACGC10550.014.7298573
TAACGCC9750.014.6102564
TCGTTTA9100.014.2307730
TAACGAA6400.014.164062513
TAGCGGC9400.013.97340330
GACGGTA9600.013.8749998
CGGACCA11700.013.7564119
Produced by FastQC (version 0.11.5)