FastQCFastQC Report
Thu 28 Apr 2016
ERR1041538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306986
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167950.5078642606893407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135680.41028295856105834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116510.35231476637639225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61060.18463942695856592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAA555.1458995E-420.18181827
AAGACGG7450.017.6308735
TAAACGC3550.016.67605828
CGTCGTA5050.016.11881310
ACCGTCG5350.015.9065438
CAAGACG8600.015.7034884
ACGGACC7850.015.554148
GACGGAC7650.015.4771247
CCGTCGT5450.015.275239
CGTTATA855.3679344E-415.2352942
CTAGCGG4450.014.96629329
TCGTTTA6100.014.86065630
TAACGCC6700.014.6343294
TACCGTC6000.014.4916677
TCTAGCG4600.014.4782628
ATACCGT6550.014.4045796
CGCATCG6450.014.34108513
ATAACGC7450.014.1543623
GCGTTAT5500.014.1272741
GTCGTAC1051.6571145E-414.0952391
Produced by FastQC (version 0.11.5)