FastQCFastQC Report
Thu 28 Apr 2016
ERR1041537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960231
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213221.087728946231337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210891.0758425920210424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139000.7091001009574892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90510.4617312959544054No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA47070.24012476080625192No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25820.13171916983253504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.12738294619358637No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.11049718119956271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG2000.017.5756
AAGACGG3200.017.343755
TAGTACC1655.4569682E-1116.8181824
TACGACG2100.016.7380945
ACGGACC3350.016.0149258
CGACGGT2200.015.9772737
GACGGTA2201.8189894E-1215.1363648
CTTAGGC1601.0977601E-815.031253
GCGAACT750.004105779314.823
CGTTAAT750.004105779314.81
ATGGTCG2550.014.50980436
GACGGAC3600.014.3888897
CGATGCG2353.6379788E-1214.17021233
CGGACCA3700.014.09
CAAGACG4500.013.97777754
CGCATCG2650.013.96226313
CGGGTTG1601.7851744E-713.87514
TATATCG1601.7851744E-713.8755
TGGTCGG2950.013.7966137
TCGTTTA2700.013.70370430
Produced by FastQC (version 0.11.5)