FastQCFastQC Report
Thu 28 Apr 2016
ERR1041536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148128
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147180.6851547021406545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT132060.6147678350638324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103850.48344418954550195No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.23387805568383263No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA28050.13057881094608886No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24510.11409934603524557No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22430.10441649659610601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3300.018.529
TACGACG3750.018.2533325
TCTAGCG3350.018.2238828
TAAACGC2100.017.61904728
CGACGGT3600.017.4722217
TTTAGCG752.068321E-417.26666626
ACGACGG3700.017.06
TAGACAG3400.016.8676475
AAGACGG6050.016.8181825
CGCAAGA6350.016.0236232
CGGTCCA4400.015.97727310
CAAGACG6500.015.9384624
GACGGTA3950.015.924058
CTACGAC4300.015.9186044
ACGGACC5900.015.6779668
GTATAGG2850.015.5789481
CGGACCA6100.015.4672149
CGCAATA3950.015.45569636
GACGGAC6000.015.4166667
GTAAACG2300.015.28260927
Produced by FastQC (version 0.11.5)