FastQCFastQC Report
Thu 28 Apr 2016
ERR1041535.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041535.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694674
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210641.242952921918906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199261.17580136356609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT153380.9050708277816264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81480.48080043713422166No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA49610.2927406687067837No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.14061701542597574No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.13412609150786522No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA20240.11943300009323327No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.11500737014906702No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA18070.1066281774547789No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.10173048031656826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA3900.019.9230772
AAGACGG4200.018.9404755
ACGGACC4150.018.722898
TTAACGG1103.8548023E-818.535
ATACCGT2550.018.1372556
GACGGAC4400.017.659097
ACGCGCG2650.017.45283121
CGTCGTA2050.017.14634310
TACCGTC2050.017.1463437
CGCGCTA2700.017.1296324
ACCGTCG2200.016.8181828
AGACGGA4800.016.5729186
CGGACCA4700.016.5319149
CTAGCGG1352.2213499E-816.44444329
GCGCAAG4850.016.4020611
CGCGCGC2850.016.2280722
CCGATGT803.3837504E-416.187514
CGAACGA1403.475907E-815.85714316
GTACTAG1302.5908776E-715.6538461
CAAGACG5100.015.5980394
Produced by FastQC (version 0.11.5)