FastQCFastQC Report
Thu 28 Apr 2016
ERR1041534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290373
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181991.41036738989424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178261.3814610194106665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138141.0705431685256899No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75420.5844821613595449No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA47740.3699705434010166No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.1861477262775957No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.16514604691821666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.14832920403635227No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA18430.14282691903813857No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA16070.12453763369196348No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15970.12376266397390523No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.11903534869374978No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14030.10872825144357486No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.10214100884007957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT500.00703533918.4999986
TCTAGCG1800.017.47222128
CGAACGA1301.3935278E-817.07692316
AAGACGG2500.017.025
CAAGACG2550.016.6862744
ACCGTGT904.4466375E-516.4444458
TACCGTC2600.016.3653857
GTATAGT1059.3447925E-615.8571441
ATAATAC1751.3096724E-1015.8571433
ATACCGT2800.015.8571436
ACGAACG1403.473724E-815.85714315
CGACGAC1302.589477E-715.65384633
ACGGACC2600.015.6538468
CTAGCAC1201.9358504E-615.4166673
CGTCGTA2650.015.35849110
CTAGCGG2055.456968E-1215.34146329
GCGGGTA1353.9729457E-715.07407323
GACGACC1353.9729457E-715.07407334
CGCAATA2109.094947E-1214.976191536
CCGTCGT2750.014.89
Produced by FastQC (version 0.11.5)