FastQCFastQC Report
Thu 28 Apr 2016
ERR1041533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296622
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT186841.4409750875737108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176561.361692150835016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134291.0356912037586898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76040.586446936732525No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA41860.32283888442429637No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41280.31836572262386414No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG34860.2688524489018388No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA27700.21363203771029646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.17591865632389392No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.16897754318529223No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG21430.16527561617803802No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.1453777585140465No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA17380.1340406070543304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.12262633211529651No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15620.12046687469439821No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13820.10658464841719482No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC13810.10650752493787705No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT13800.10643040145855924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG358.8700117E-426.428579
CGCATCG2550.018.86274513
CCGGTCG2400.018.520
ACGAGTC609.2376873E-418.526
GACCTAG801.61693E-518.51
GTAAACG1501.2732926E-1118.527
CGAACGA1002.8756222E-718.49999816
AAGACGG2500.017.765
ACGAACG1054.7984395E-717.61904715
TCGTTTA2650.017.45283130
GACGGAC2450.017.3673467
TAACGCC2800.017.1785724
AATAACG3150.017.0317462
ATTATAC1201.0414806E-716.9583323
TCGCCGG2850.016.87719317
AACGCCG2850.016.8771935
GGCTAGA1005.878961E-616.651
GCGCAAG2800.016.5178571
GCATCGC3250.016.50769214
TTATACC1708.54925E-1116.3235284
Produced by FastQC (version 0.11.5)