FastQCFastQC Report
Thu 28 Apr 2016
ERR1041532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181565
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT245571.12565979010481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT242871.113283353922528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT184850.8473274919610464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89830.41176861564977435No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA48820.22378430163666907No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31180.14292491857909345No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG28300.12972338665132602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.12830238842298994No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.11794285295189462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC551.9036748E-523.5454543
TACACGC656.905763E-519.9230775
GACGGTA3300.019.0606088
CGACGGT3350.018.776127
TACGACG3500.018.55
ACGGTAT3450.018.2318849
CTACGTA852.724931E-517.4117649
ACGACGG3650.017.2328766
TCTAGCG1851.8189894E-1217.00000228
CGGTCCA2550.015.96078410
GATCGCG700.002593495215.85714218
TAACGGC1201.937342E-615.41666636
CTAGCGG1802.0190782E-1015.41666629
CTACGAC4450.015.3820224
GTCCGGA1101.4529782E-515.13636433
CGCAAGA4100.014.8902442
TGCGACG750.00410597714.822
AAGACGG3950.014.5189875
GACGGAC3950.014.5189877
TATACTG4500.014.3888895
Produced by FastQC (version 0.11.5)