FastQCFastQC Report
Thu 28 Apr 2016
ERR1041531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291562
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91590.2782569491323572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81340.2471167184455283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56490.17162064697550888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.10359823087032842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG7450.018.375845
TTAACGG2100.017.61904735
ACGGACC6650.017.248128
GACGGAC6750.016.9925927
CGCAAGA7500.015.7866662
TAACGCC4350.015.7356314
ATTAGAG6900.015.2826083
CGCATCG4550.015.04395613
CGAACGT2006.184564E-1114.7999994
CGGACCA7650.014.7516349
ATCGCCG5150.014.72815616
TATACTG6950.014.6402875
TAACGGC2550.014.50980436
AACGCCG4600.014.4782615
AGACGGA8350.014.4011976
ATGGTCG4650.014.32258136
ATAACGC5850.014.230773
CGTACGG1304.4497374E-614.23076922
AATAACG4850.014.1134022
GCAAGAC12750.013.9294123
Produced by FastQC (version 0.11.5)