FastQCFastQC Report
Thu 28 Apr 2016
ERR1041530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834372
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39530.4737694937030485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.3970650980617758No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.17450250008389545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12820.1536484925189244No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10960.13135627753567955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC2200.026.909098
GACGGAC2350.025.191497
AAGACGG2950.022.5762715
CGCAAGA2700.021.9259242
CGGACCA2850.020.7719299
GTATACG450.003824942320.5555551
AGACGGA2950.020.0677976
GGACCGT656.8992325E-519.9230776
GCGCAAG3100.019.6935481
CAAGACG3250.019.3538464
GGTATCA14800.019.1251
CCGATCG609.233921E-418.514
CGATCGC609.233921E-418.515
TCGCGTA1800.018.59
AGCGAAA3450.017.69565217
GGCGTTA1156.398659E-817.6956531
GCGAAAG3350.017.67164218
TACCGTC1705.456968E-1217.4117647
CGAAAGC3300.017.37878819
TCGCACG752.0664105E-417.26666618
Produced by FastQC (version 0.11.5)