FastQCFastQC Report
Thu 28 Apr 2016
ERR1041529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519761
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62990.41447306517274757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61670.40578748895385525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39950.26287028026117265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.1506157876139735No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15400.10133172255374365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG656.9040005E-519.9230775
AAGACGG3750.017.7599985
CGAGCCG3350.017.67164215
CTAGCGG1603.45608E-1117.3437529
GACGGAC3900.016.6025647
TACCGTC2800.016.5178577
GCGCAAG4050.016.4444451
TCGTTTA2750.016.14545430
CGTCGTA2750.016.14545410
ACGGACC3900.016.1282068
TATACTG4050.015.9876555
CGACGGT2550.015.9607847
ACACCGC700.002593009715.8571426
ATAGACG700.002593009715.8571423
CAAGACG4200.015.8571424
GCGTAGA700.002593009715.8571421
GCGATGC2250.015.62222232
CGCAATA2250.015.62222236
AACGCCG2850.015.5789475
CCGTCGT2900.015.3103459
Produced by FastQC (version 0.11.5)