FastQCFastQC Report
Thu 28 Apr 2016
ERR1041528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375001
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138320.40983691560387686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125960.3732147042326802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94580.28023695400386545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46620.1381332924049504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9050.017.171271
GACGGAC7250.016.5862067
TTGACGG7150.016.300733
ATTGACG7050.016.26950332
ACGGACC7550.016.1721868
CTAGCGG4350.016.16092129
CGCAAGA8000.015.9562512
TCTAGCG4450.015.79775228
TAGACCG957.0661525E-515.5789485
CGTATTA1455.3552867E-815.31034515
GGTATCA68000.014.7183831
AAGACGG8550.014.713455
ATAACGC6600.014.5757573
TCGTTTA5100.014.50980430
ACCGTCG5500.014.4636358
CGGCGAT6700.014.35820930
TAACGCC5300.014.3113214
CGCATCG5200.014.23076913
CGCAATA4750.014.02105236
CGATGCG6600.014.01515133
Produced by FastQC (version 0.11.5)