FastQCFastQC Report
Thu 28 Apr 2016
ERR1041527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104761
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32633.1147087179389277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24282.3176563797596432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14801.4127394736590908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7150.6825058943690877No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.37323049608155706No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.3379120092400798No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.32263915006538696No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2600.2481839615887592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2580.24627485419192255No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.23959297830299445No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2500.2386384246045761No Hit
CATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAA1950.18613797119156938No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1810.17277421941371313No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.15177403804851042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1520.1450921621595823No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA1480.1412739473659091No Hit
CATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1390.13268296408014432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1300.1240919807943796No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA1300.1240919807943796No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG1290.12313742709596127No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA1290.12313742709596127No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG1230.11741010490545147No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1220.11645555120703315No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.10786456792126842No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA1120.1069100142228501No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG1100.1050009068260135No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1090.10404635312759519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGAC308.2400275E-637.03
GCGCAAG355.566435E-737.01
CGCAAGA401.5817641E-632.3752
AAGACGG352.3677976E-531.7142855
GACGGAC352.3677976E-531.7142857
CAAGACG352.3677976E-531.7142854
AATCCCA250.005473848429.66
ACGGAAG250.005473848429.636
ACACTGA250.005473848429.66
AGTAGTA250.005473848429.62
GTGGTAC250.005473848429.63
AGACGGA405.889524E-527.7500026
ACGGACC405.889524E-527.7500028
CGGACCA405.889524E-527.7500029
GAGCGAA451.3120304E-424.66666616
CAGAGCG451.3120304E-424.66666614
GGGTGGT400.001919556423.1250021
GACCAGA502.6794523E-422.211
AGCGAAA502.6794523E-422.217
GCGAAAG555.1003357E-420.18181818
Produced by FastQC (version 0.11.5)