FastQCFastQC Report
Thu 28 Apr 2016
ERR1041526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913106
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170151.8634200191434511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161961.7737261610371633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66700.7304737894614645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63030.6902813035945443No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA25200.2759811018655008No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23300.2551730029153242No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20350.22286569138741832No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20310.22242762614636197No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18240.19975774992169584No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17950.1965817769240373No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.17226915604540985No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.15726542153922984No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT13400.14675185575387742No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.14576620896150064No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12070.13218618648875377No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11000.12046794129049639No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10320.11302083219253843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA250.005495759729.5999989
GGGCGCA500.00703380218.49999811
CGTCGTA2000.017.57499910
TACCGTC2000.017.5749997
CTACACC953.604744E-617.5263164
GGTTATC1603.45608E-1117.343752
TTATAGT1005.8751284E-616.654
TCTTATA3150.016.44444537
TTGCGCG904.444347E-516.44444518
CCGTCGT2150.016.3488379
ACCGTCG2150.016.3488378
ACGGACC2650.016.0566048
TTAATAC1403.4699042E-815.8571423
TAGACTG700.00259194715.8571425
ACTAGAC700.00259194715.8571423
CCGTTCA700.00259194715.85714236
TGACCCG1659.731593E-1015.6969713
GTCGTAG2250.015.62222211
CAAGACG3000.015.4166674
GTTATCT1802.0190782E-1015.4166663
Produced by FastQC (version 0.11.5)