FastQCFastQC Report
Thu 28 Apr 2016
ERR1041525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10637715
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1019530.9584107113228734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT722300.6789992023662977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT553640.5204501154618261No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489660.46030562014492776No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG315920.2969810715929126No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG201540.18945798040274625No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT200480.18846152580699896No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG193090.18151454518193053No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA170330.16011897291852623No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153880.14465512565433458No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT151150.1420887850445326No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT146650.13785855327013366No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG146160.1373979280324769No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138350.13005612577513123No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG132070.12415260232108118No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127860.12019498548325462No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT123430.11603055731423526No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG116720.10972281171285375No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA114980.10808712209341949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT12400.021.0362937
CCCACGA14150.019.08833919
ACGAGAC15550.017.01286322
ATCTCCG16900.016.52958710
TCTCCGA17700.015.99152511
CGAGCCC17700.015.88700515
GCCCACG19550.015.04603718
CGTTCGG2600.014.23076924
CTCTAAT25600.013.3691411
CACGAGA21000.013.30238121
TAACGGC21000.012.9536
GTATTAG38650.012.8279441
TTAACGG21300.012.68075135
ATTAGAG27000.012.6074083
GATCTCG21800.012.55963336
ATACTGG30050.012.4975056
AATACTG31150.012.412525
CTAATAC36300.012.3842973
TAATACT41500.012.3036154
TACTGGT27550.012.1542657
Produced by FastQC (version 0.11.5)