FastQCFastQC Report
Thu 28 Apr 2016
ERR1041524.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041524.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7124396
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT842701.1828371134900417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT622690.8740249699764021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT473390.6644633453839455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409110.5742381529606159No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG212980.29894464035968804No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134530.1888300425748372No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124040.17410598737071886No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG123840.1738252618186861No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT123590.17347435487864515No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123310.1730813391057993No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA117770.16530524131449179No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG115480.16209093374371666No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT100800.14148567822451194No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG88040.12357538800482175No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT86310.12114711197973836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.11776436907774358No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA75730.10629673027720526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT8300.017.83132637
TTAACGG11350.015.1585935
CCCACGA10050.014.91044819
ACGAGAC10750.014.28372222
TAACGGC12100.014.219007536
GTATTAG22050.014.0952391
ATCTCCG11150.013.771310
GGACCGT1907.1449904E-913.6315796
CGAGCCC11750.013.38297815
CACGAGA12200.013.34426221
TCTCCGA11650.013.18025711
CCGAGTT6800.013.05882313
ATTAGAG17150.013.0524783
TAATACT27000.012.470374
CATCTCG12350.012.43319836
GTTCTAG7450.012.4161071
GCCCACG12550.012.3824718
CTAATAC23300.012.0686693
GACCGTG4300.012.0465127
GCCGAGT6800.011.97058812
Produced by FastQC (version 0.11.5)