FastQCFastQC Report
Thu 28 Apr 2016
ERR1041523.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041523.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8284854
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT258750.312316909869504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT223320.26955212487751745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182450.22022114089155945No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT105310.12711147353954577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92750.11195127880346473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61550.027.8627131
CGAGACA21650.021.27713623
ATCTCGT21550.020.94663637
CCCACGA23650.019.24312819
ACGAGAC24800.018.64919522
ATCTCCG24800.018.42540410
CACGAGA25850.018.03481721
AATCTCG25400.017.77165436
CGAGCCC27200.017.0716915
TATACAC30150.016.9966833
GTATCAA101150.016.9545232
TCTCCGA27650.016.92766811
GCCCACG28650.016.27225118
CCACGAG29350.015.82112520
CCGAGCC31200.015.71314114
TACACAT34950.015.2975665
TCCGAGC31200.015.23878313
ACACATC35900.014.0682456
ATACACA41400.013.6739134
AAATCTC33700.013.61424335
Produced by FastQC (version 0.11.5)