FastQCFastQC Report
Thu 28 Apr 2016
ERR1041522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6533552
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT395820.605826662128043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT309580.4738310799393653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224430.3435038092602615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180250.2758836234868874No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG131030.20054941018300612No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG84160.1288120152713256No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG82560.1263631176425932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG1752.244633E-914.85
TTAACGG8550.014.06432735
TAACGGC9050.013.287292536
GGCGAAT3300.012.33333432
CTACACT11850.012.1772154
GTATTAG19150.012.0757181
TATACTG9700.012.0154645
ATTAGAG15550.011.6591633
CTCTAAT15000.011.5933341
TAATACT20950.011.5680194
TACCCCG7050.011.54615
TTAGGAC11700.011.5427343
CTAATAC19800.011.4924243
GTAATCG1951.964152E-611.38461420
AATACTG19050.011.3622055
GCGAATC3750.011.34666733
GGGTAAG9400.011.2180841
CGGGTAT2655.3696567E-911.1698116
ACGGACC4150.011.1445788
TAGGACC11800.011.1313564
Produced by FastQC (version 0.11.5)