FastQCFastQC Report
Thu 28 Apr 2016
ERR1041521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8201842
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1751822.1358860607166053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT884201.0780505159694616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT783030.9547001758873189No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG774320.9440806101848829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT774260.944007455886129No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG437620.5335630703444422No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG404890.49365740037420863No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA381830.46554176488647303No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT356790.4350120375398599No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG313300.38198736332643324No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT301770.3679295455825655No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA287310.35029935958288394No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253620.3092232208325886No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG237200.2892033277402808No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234120.28544807373758235No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT230430.28094908436421967No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT215280.26247762392886864No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA209330.2552231559691104No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203230.24778580226246738No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG200440.24438412737041265No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA189320.23082619733469628No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180410.2199627839697473No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT177140.21597587468766163No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA176720.21546379459638454No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA174690.21298874082187877No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG164980.20114993680687823No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG163010.19874803733112636No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG156830.19121314455947822No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC156710.1910668359619705No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC141630.17268072220849903No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA131740.1606224552972369No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA121280.14786922254781304No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108440.13221420261448588No Hit
GTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA107750.13137292817881643No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA107470.1310315414512984No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT106010.12925145351495432No Hit
GGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA105350.1284467562286618No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG101640.1239233820890478No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT98930.12061924626199823No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA97700.11911958313754398No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC92860.11321846970473218No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG91750.11186511517778568No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA90550.11040202920270838No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG90500.11034106728708014No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA89480.10909744420826444No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA88260.10760997346693583No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87290.10642731230374836No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC86940.10600057889435081No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT86600.10558603786807891No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA84790.1033792165223373No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA82770.10091635513095717No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82770.10091635513095717No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT82550.10064812270219299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA18050.025.3157925
CGAGACG18950.024.01583123
GACGTAG19450.023.77892326
ACGAGAC19450.023.49357622
CCCACGA19950.023.09022519
AATCTCG19850.023.02015136
ATCTCGT19850.022.83375437
CGTAGAG20350.022.81818228
GAGACGT20350.022.63636424
ACGTAGA20300.022.4187227
CACGAGA21000.021.75952321
TCCGAGC22200.021.2513
GCCCACG22500.020.30888718
TCTCCGA23250.020.13118211
CGAGCCC23050.019.98481615
ATCTCCG23300.019.92918410
CCACGAG23250.019.81290220
CCGAGCC24800.019.3951614
GAATCTC23600.019.28389735
CTCCGAG25500.018.512
Produced by FastQC (version 0.11.5)