FastQCFastQC Report
Thu 28 Apr 2016
ERR1041520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7946681
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1822122.2929321058691046No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG1110571.3975268417091362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT913881.1500147042520015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT859571.0816717067163009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT815551.0262775113283142No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG619550.779633660895662No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT545780.6868024524955765No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG527900.6643024930785569No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG460080.5789586872808912No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA419240.5275661625274753No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA415860.5233128144945041No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT400370.5038203999883725No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG359420.4522894526658362No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338910.42647993545984797No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324200.40796906280747897No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT318590.40090951178234036No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG308000.3875831935370251No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA298100.37512516231619214No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270460.34034334585722015No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT262980.33093061115703526No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA259800.3269289405224647No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT248700.3129608449112277No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG233030.29324192074653554No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC228880.28801961472972176No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA228440.28746592445324026No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG227650.28647179872955764No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG224410.28239462487546685No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211300.2658971714103032No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC197560.24860693414017748No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA193340.24329654103392348No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA164600.2071304988837478No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA162390.20434946363142048No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT160590.20208436704581448No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT154610.1945592128336346No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG150050.18882096815009938No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG145590.18320856216576456No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC142100.17881679156367294No Hit
GGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA136720.17204666954669504No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG131200.16510037335083666No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA129000.16233192196842933No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126460.15913561900874088No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC121940.15344770980488584No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT120080.15110710999975965No Hit
GTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA119010.1497606359183161No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC118480.14909369081255433No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT117820.1482631553978321No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA114340.14388396866566053No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC111070.13976904320180966No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA109220.1374410272666035No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA107300.1350249242419571No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106810.1344083146158755No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT105010.1321432180302695No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC103420.1301423827129842No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA103100.12973969887554315No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA103010.12962644404626283No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101830.1281415473956989No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA101520.12775144742817787No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA100660.126669234615055No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA95560.12025146095583804No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA95410.12006270290703754No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCT95310.11993686420783721No Hit
GTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAA94410.11880431591503422No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT94050.118351296597913No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCC93960.11823804176863272No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA89400.11249979708509754No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88800.11174476488989553No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG86420.10874980384892761No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA85200.10721457171868355No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC85040.10701322979996303No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCT84900.10683705562108256No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA84420.10623302986492097No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGAT82880.10429511389723585No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC82730.10410635584843535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT23600.026.33898437
CGAGACA24250.026.31958823
AATCTCG24250.026.09072136
ACGAGAC24800.025.95967722
CCCACGA26000.024.7615419
CACGAGA26300.024.47908821
CGAGCCC27250.023.55779815
GCCCACG27650.023.35081518
CCGAGCC28400.022.79929514
TCCGAGC28100.022.7793613
GGCAATC28150.022.6074633
CCACGAG28650.022.6003520
AGGCAAT28500.022.4596532
GCAATCT28400.022.40845134
ATCTCCG29200.021.85787610
TCTCCGA30100.021.5116311
CTCCGAG30450.021.02134712
GAGACAA30500.020.9262324
ACAAGAG30650.020.82381627
AGCCCAC31600.020.72468417
Produced by FastQC (version 0.11.5)