FastQCFastQC Report
Thu 28 Apr 2016
ERR1041519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5138132
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1003621.953277961718383No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG763851.48662977128653No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG497340.9679393211384995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT453180.8819936895354187No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421140.8196363970407923No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT405220.7886523740534498No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG365980.7122822068409297No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG342540.6666625147037873No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA324360.631280006041106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT312390.6079836018226079No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG308110.5996537262958601No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT278620.5422593269304876No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA244950.47672967529833804No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT215780.41995807036487187No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA211550.4117255064681094No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA211310.4112584106441796No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG193770.3771214908453111No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG179420.3491930530395093No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC167980.3269281520988562No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT153310.298376919861148No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG145290.2827681344114943No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG141150.2747107314487055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT139830.2721417044170917No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC134790.26233269211456617No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134090.260970329294771No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT133030.25890732273908107No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC126420.24604272525501486No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113650.2211893349567508No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA110640.21533117483163142No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG109760.21361849014388887No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104290.20297259782348914No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT100610.19581046185656578No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG100550.19569368790058334No Hit
GGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA100130.1948762702087062No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA90130.17541394421163178No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT84730.16490428817321157No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT83970.16342515139743394No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA83030.16159569275370894No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA80870.15739183033834087No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA80830.15731398103435257No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG80180.15604892984454272No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT79630.15497850191470364No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA78750.1532658172269611No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG78500.1527792590770342No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC78300.15239001255709272No Hit
GGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA76850.14956797528751695No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC73240.14254207560257307No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC73230.142522613276576No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC69530.13532155265765847No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT69180.13464037124776085No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA67630.13162371071821433No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67320.131020378612305No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC66160.1287627487966444No Hit
GAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA63800.12416963986133481No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCC62960.12253480447758057No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC62170.1209972807238117No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61620.11992685279397258No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA60850.11842825369219787No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA59100.11502234664270984No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC56960.11085740887933591No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA56170.10931988512556705No Hit
AACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG53960.10501871108021359No Hit
ACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC53600.10431806734431891No Hit
GCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA53290.1037147352384096No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCT53140.10342280034845348No Hit
GCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG51980.10116517053279286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGA14150.023.6643125
ACGAGAC14150.023.6643122
ATCTCGT13950.023.34050237
GAGACCG14350.023.33449624
CCCACGA14600.023.06164619
CACGAGA14650.022.85665521
GATCTCG15000.022.44666536
TCTCCGA15350.022.41693711
GCCCACG15550.022.12861618
CGAGCCC15750.021.84761815
ATCTCCG15850.021.4763410
ACCGAGG15650.021.27795627
TCCGAGC16300.020.99693113
GACCGAG15950.020.99372926
CGAGACC16300.020.54294423
CCGAGCC16900.020.2514814
CGAGGCT16950.019.97345229
CCACGAG17800.019.3314620
AGCCCAC18100.019.2154717
CTCCGAG17950.019.16991612
Produced by FastQC (version 0.11.5)