FastQCFastQC Report
Thu 28 Apr 2016
ERR1041518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394090
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368940.6839707902537777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT244520.4533109384530106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195710.36282301555962176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160700.2979186480017946No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG143000.26510495746270457No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG93610.17354178369289353No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG81270.15066489435660138No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA71420.1324041682656389No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT68320.12665713771924458No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT67890.12585996896603505No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG58200.10789586380649933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT17150.014.1311951
TAATACT26000.013.8754
TTAACGG12000.013.72083435
AATACTG21850.013.7162475
GTATTAG20650.013.4382561
TAACGGC12450.013.37349436
ATTAGAG15350.013.2573293
ATACCGA1551.891005E-613.1290326
TACTGGT19500.012.9974367
CTACGCT2900.012.7586230
AACGGCC13750.012.51272737
GGTAAAC17300.012.40462435
ATACTGG21950.012.3895236
CTAATAC22750.012.3604393
CGAACGA1653.8103117E-612.33333314
GGGTACG1807.4773743E-712.3333331
GTATACG2859.094947E-1212.3333331
TTAATAC6800.012.2426483
TACTCGC4100.012.18292720
GATGCTA18550.012.16711614
Produced by FastQC (version 0.11.5)