FastQCFastQC Report
Thu 28 Apr 2016
ERR1041517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6812557
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT126410.18555441077410434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122290.1795067549526558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90450.1327695313228205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.025.6083761
GTATCAA64000.016.18752
ACCGTCG2551.8189894E-1213.78431323
ATCAACG94150.012.084442
CAACGCA95050.012.0089434
TAATACT17700.011.9152544
TCAACGC95650.011.894933
GTATTAG14300.011.7727271
TTAGACT6050.011.6198354
CTCTAAT10400.011.5624991
AACGCAG99650.011.5474165
TTAACGG5450.011.20183535
CTAGTAC6650.011.127823
TCTATAC8150.011.1226993
AACGGTA2502.7206625E-811.09999918
TAGACAG12700.011.0708675
CTAATAC13750.011.0327273
TAGACCG1350.001376481110.9629635
CGGTTAT1350.001376481110.9629631
CTACACT13700.010.9379564
Produced by FastQC (version 0.11.5)