FastQCFastQC Report
Thu 28 Apr 2016
ERR1041516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5477074
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180280.32915385112561923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136880.2499144616267737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91800.16760774092152123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA1005.885926E-616.6510
TTAACGG7100.015.89436735
TAACGGC7350.015.35374236
CGCGATA750.004107030614.814
GGTATCA77550.014.4803351
GTATTAG14700.013.7176881
ATTAGAG10800.013.0185183
TTTAACG8900.012.8876434
CTAATAC13950.012.8637993
TAGACAG9500.012.4631585
AGGTCGC9700.012.39690735
GCTAGAC3650.012.1643843
TAATACT15250.012.1311474
CGAACTA8750.012.05142924
TATACTG10100.011.9059415
AACGGCC10400.011.562537
GCGCTAG1300.001003977611.38461511
GTGTAAG10300.011.3155341
AACGCAG129250.011.1930375
TGTACTG22200.011.1666675
Produced by FastQC (version 0.11.5)