FastQCFastQC Report
Thu 28 Apr 2016
ERR1041515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5985430
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT519490.8679242761171713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT328980.5496346962540704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT232120.38780839471850814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209980.3508185710968134No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG84580.14130981399832593No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67030.1119886123469826No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64260.1073607075849187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT8400.012.7738094
TTAGACT6350.012.236224
CTAGTAC6350.012.236223
GTATTAG8500.011.9705891
ATCTCGT7050.011.80851137
CTACACT11400.011.684214
TTATACC9200.011.6630444
CTAATAC11100.011.53
TAGACAG8700.011.4827585
CCTAGTA6300.011.452382
TAGACTG6000.011.4083335
GTACTGT14500.011.2275866
TATACCG4450.011.2247185
TATACTG10200.011.0637255
GTACCGT2707.192284E-910.9629626
TAATACC7650.010.8823534
TATACAC11950.010.8368213
TATACAG9750.010.8153855
GTATACG6000.010.7916661
ACGAGAC8100.010.73456822
Produced by FastQC (version 0.11.5)