FastQCFastQC Report
Thu 28 Apr 2016
ERR1041514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6375414
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT203900.3198223676140875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154380.24214898044268182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108780.17062421358048277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66300.10399324655622365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.016.366731
TATACTG10000.013.1349995
AACGCAG135600.012.8790555
TTAACGG4600.012.06521835
ATACTGT14050.011.9822066
GTATCAA111450.011.6527592
ACGCAGA152550.011.5086866
CGCAGAG157800.011.149247
CTAGTAC5900.010.9745763
ATCAACG163450.010.8657082
CAGAGTA165950.010.7243159
AGAGTAC163400.010.71052710
TCAACGC166000.010.6430713
TAGACCG1759.3807874E-510.5714295
CAACGCA166700.010.5539894
GTATTAG13400.010.49253751
TATCAAC166200.010.4855591
TAACGGC5350.010.37383236
AATACTG13750.010.365
ATTAGAG10200.010.3382363
Produced by FastQC (version 0.11.5)