FastQCFastQC Report
Thu 28 Apr 2016
ERR1041512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439623
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175630.3228716401853584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130300.23953865920487505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86700.15938604568735737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75300.015.9448871
TAGGTCG3800.015.57894821
TTAACGG4700.014.17021435
CTAGTAC5700.013.6315783
GTACTAG3950.013.1139251
GTATTAG12600.013.067461
AACGCAG118950.012.6132835
TAGACAG9000.012.1277785
AACCGTA2307.928975E-912.0652187
TAGTACT9300.011.9354844
GTATCAA101600.011.7627952
ACGCAGA129900.011.56428056
TATACCG3053.092282E-1111.524595
ATCAACG130750.011.4042062
CGCAGAG131650.011.3965057
ATTAGAG8850.011.2881363
CAACGCA133450.011.2150624
TCAACGC134050.011.1924653
CAGAGTA135800.011.170849
TAATACT11600.011.1637934
Produced by FastQC (version 0.11.5)