FastQCFastQC Report
Thu 28 Apr 2016
ERR1041511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5187800
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93610.1804425768148348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81280.15667527661050928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59280.1142680905200663No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC58400.1125718030764486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.020.4739461
TATACAC7950.016.9874213
CTCTAAT13400.016.8432831
GTACTAG3850.015.8571441
CTAATAC22250.015.0494383
TTAACGG15250.014.67868935
TCGCTAA3550.014.59154914
AATACTG16600.014.3765065
TCTAACG2450.014.3469382
ATTAGAG16350.014.256883
TAACGCC2600.014.2307694
TAACGGC15500.014.20322736
TCTAATA18400.014.1766312
TAGGTCG3800.014.11842221
TTTAACG16250.014.00307734
GTATTAG24500.013.8183661
CTAGTAC5250.013.7428573
GTCTATA2700.013.7037031
TCTATAC6350.013.6929123
ATCTCGT8650.013.47398837
Produced by FastQC (version 0.11.5)