FastQCFastQC Report
Thu 28 Apr 2016
ERR1041510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11526683
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT541550.46982293171418005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT453750.3936518424250931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT381730.3311707279535665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198220.17196621092121644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169050.020.0375631
GTATCAA246200.013.6758732
TTAACGG7950.012.10062935
TATACTG19350.011.1860475
AACGCAG304050.010.9764847
GTCGTAC3851.8189894E-1210.5714281
TAACGGC8950.010.33519636
GTATTAG27050.010.25878051
TATCAAC336500.010.1598823
ATCAACG332550.010.1025424
CGCGATA1656.4093503E-410.09090912
TCAACGC335700.010.0297895
GAGTACG185700.010.0220791
AGTACGG185100.09.9646142
CAACGCA340150.09.8985756
TACGGGT53350.09.7788194
TAATACT27600.09.7192034
GTAAGAC11350.09.616743
TAACGCC4055.4569682E-119.5925924
CTACACT19550.09.5575454
Produced by FastQC (version 0.11.5)