FastQCFastQC Report
Thu 28 Apr 2016
ERR1041509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12856762
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT678290.5275745168184649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT539720.41979465747285355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT476200.3703887495156245No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257690.20043149278177508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166550.024.3149221
GTATCAA258600.015.624132
GTATTAG27150.012.3333341
ATCAACG355400.011.3373664
TATCAAC363250.011.2196843
TCAACGC363550.011.1035625
AACGCAG361400.011.09283357
TTAACGG8600.010.9709335
CAACGCA368450.010.96091656
CTAGTAC11150.010.2869953
TATACTG22900.010.2598255
TAATACT35450.010.2284914
GAGTACG208350.09.9980791
TAACGGC9900.09.9040436
AGTACGG209250.09.8666682
ACGCAGA416250.09.6444448
GTATAGG17750.09.3802811
CTACACT22150.09.3544024
TACACTG31050.09.3542675
CGCAGAG427600.09.3451829
Produced by FastQC (version 0.11.5)