FastQCFastQC Report
Thu 28 Apr 2016
ERR1041508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7759962
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT366270.4719997340193161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT327940.42260516224177386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT264580.3409552778737834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146900.1893050507206092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144100.015.8039561
GTACTAG5950.012.4369751
GTATCAA198050.011.4241362
TATACTG14400.011.3055555
AACGCAG208800.010.6144647
TAGAACA12850.010.50972754
TATACCG3208.6765795E-1010.406255
TACGCTA4100.010.3780531
ACGCTAT3953.6379788E-1210.30379832
GAGTACG124450.010.197671
CTAGTAC6750.010.1407413
AGTACGG124550.010.0855072
TCGCGTA2402.147799E-610.02083311
CTATACT14750.09.9084754
TTAGGAC9550.09.8795813
CTACGCT4500.09.86666630
GTATTAG13650.09.7582421
TCTATAC9900.09.7171723
GACGCTA4403.6379788E-129.67045426
TTAGACT7850.09.662424
Produced by FastQC (version 0.11.5)