FastQCFastQC Report
Thu 28 Apr 2016
ERR1041507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7044980
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT338500.48048397582392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT296530.4209096406235362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237380.33694914676833715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125740.17848169902540534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101000.020.1301981
GTATCAA148650.013.7023212
TTAACGG4300.013.33720935
TAACGGC5050.011.35643536
AACGCAG185300.010.8024837
GCGTAAG1901.8297073E-510.7105261
GAGTACG110300.010.3485951
TAATACT17450.010.2836684
TCCTATA5950.010.2605052
AGTACGG110300.010.1976432
TATCAAC203500.010.1909093
TTTACAC14950.010.1471573
TACTCCG9300.010.1451625
ATCAACG200150.010.1304034
TAGTACC6400.010.11718754
GTACTAG5150.010.0582531
TATACTG11850.09.9915615
TCAACGC202100.09.9777345
CTAGACT6700.09.9402984
GTATTAG15650.09.9297121
Produced by FastQC (version 0.11.5)