FastQCFastQC Report
Thu 28 Apr 2016
ERR1041505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110033
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT518370.5690100134653738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT502720.551831151434907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT359700.3948394039845959No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212920.23372033888351446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208750.015.8634731
TCTATAC10700.012.1028033
GTATCAA287300.011.55847552
CTACACT15400.011.5324674
TCTAGAC10100.011.3564353
TAGACCG2301.0258191E-711.260875
TATACTG15300.011.1241835
CTAACGC2259.382147E-710.6888893
AACGCAG308450.010.6519695
TAGACAG14800.010.6250015
GTATTAG20900.010.6220091
GTACTAG6450.010.0387591
GAGTACG186400.09.815721
AGTACGG185550.09.72112
GGACCGT5950.09.6386566
TCTACAC16600.09.4728923
GTACTAA6500.09.3923071
ACGCAGA353400.09.3337586
TAGGGGT9950.09.2964824
CTAGACA14150.09.2826854
Produced by FastQC (version 0.11.5)