FastQCFastQC Report
Thu 28 Apr 2016
ERR1041504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6095565
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323620.530910588271965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291970.47898759179829925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT228530.37491192366909387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125880.20651079924502488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103850.019.5777571
GTATCAA150500.013.3863782
CGAACCG1205.127053E-412.3333345
TATACTG11100.012.05
GTATTAG12550.011.940241
GTATAGG8200.011.2804871
AACGCAG186500.010.7627347
GAGTACG116600.010.5986281
TAATACT14850.010.4646464
AACGGTA2301.2443779E-610.45652218
AGTACGG116500.010.4489262
AATACTG14050.010.4021365
TTAACGG4055.456968E-1210.04938235
TATCAAC205150.010.0277843
ATCAACG204150.09.9681614
TCAACGC207050.09.8017395
ATCGTAC1708.429636E-49.79411825
TTATACT13600.09.7941184
ATACTGT13250.09.7735846
TTAGGAC8750.09.7257153
Produced by FastQC (version 0.11.5)