FastQCFastQC Report
Thu 28 Apr 2016
ERR1041503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10756754
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1279561.1895410083748312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1239291.1521040641070717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT891730.8289954385867706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT577110.5365094339798048No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190120.1767447689144885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164540.1529643608099618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT162230.15081687282241463No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159350.1481394852015766No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131650.1223882223205997No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA113760.10575681102310232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT9750.016.88717837
GAGACGT10800.015.41666724
CCCACGA11150.014.76681619
CGTAGAG11150.014.60089728
GGTATCA558000.014.193281
ACGAGAC12800.013.44140622
AGACGTA13400.013.11567225
GACGTAG12900.013.05038726
CGAGCCC12800.013.007812515
ATACCGA4000.012.9500016
TGCGACG2900.012.7586222
CGAGACG13650.012.60439623
ACGTAGA13650.012.33333327
TACCGAC3500.012.1571447
ATCTCCG13900.012.11151110
GTATTAG38900.011.9845761
CGCATCG1705.3195654E-611.97058813
TTAACGG18650.011.70509435
TCTCCGA13900.011.57913811
TAATACT46900.011.5181244
Produced by FastQC (version 0.11.5)